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Biacore
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GE Healthcare
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Cytiva Europe
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Biacore
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Chemie GmbH
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GE Healthcare
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Biacore
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Cytiva Europe
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Image Search Results
Journal: Scientific reports
Article Title: Novel anticancer agent, SQAP, binds to focal adhesion kinase and modulates its activity.
doi: 10.1038/srep15136
Figure Lengend Snippet: Figure 3. SPR analysis of the binding between SQAP and target proteins. Sensorgrams obtained by SPR analysis of SQAP (left) and SQP (right) with the candidate proteins: (A) SCP-2 KD 15.6 ± 2.2 μ M, (B) MFE-2 KD 14.2 ± 1.79 μ M, (C) ADRM1 KD 9.65 ± 2.91 μ M, (D) HR23B, (E) FAK KD 3.24 ± 0.21 μ M. The candidate proteins were immobilized onto the surface of a CM5 sensor chip. A solution of SQAP or SQP at variable concentrations was injected to generate result binding responses (RU) recorded as a function of time (sec). The results were analyzed using BIA evaluation 4.1.
Article Snippet: The proteins were first immobilized on a CM5 sensor chip of the
Techniques: Binding Assay, Injection
Journal: Methods in Molecular Biology
Article Title: G Protein-Coupled Receptor Screening Assays
doi: 10.1007/978-1-4939-2336-6
Figure Lengend Snippet: Fig. 1 Procedure followed to generate lentiviral particles (LVP). Cells are transfected with the GPCR of interest (pGPCR), followed by transfection with lentiviral plasmids (psPAX2, pLVTHM, pMD2G). During budding, LVP incorporate membrane fragments that include the transfected GPCR in its native context
Article Snippet: We use two types of SPR biosensors for
Techniques: Transfection, Membrane
Journal: Methods in Molecular Biology
Article Title: G Protein-Coupled Receptor Screening Assays
doi: 10.1007/978-1-4939-2336-6
Figure Lengend Snippet: Fig. 4 Scheme showing LVP immobilization to the biosensor surface self-assembled monolayers (SAM). GPCRs expressed on the LVP surface are immobilized to the sensor surface through interaction with functional groups on SAM
Article Snippet: We use two types of SPR biosensors for
Techniques: Functional Assay